Lady Gaga has worn many, many, many looks over the course of her eye-popping career, but her newest makeover, in the new "Machete Kills" trailer, might be her most unexpected to date.

In the [article id="1708221"]latest trailer[/article], which premiered Friday (August 20), a gun-toting Cuba Gooding Jr. appears on the screen, saying, "You've seen my face."

Gooding Jr. then peels off the synthetic face to reveal Gaga lurking underneath the surface, wearing a strapless red outfit, complete with bright-yellow blond hair, looking a bit like her killer [article id="1633722"]"Telephone" alter ego[/article].

"Muy bien, indeed," says Gaga, who will play [article id="1690633"]Lady Chameleon[/article] in the flick. The camera then closes in on Gaga's face as she offers up sultry gaze.

The film, from director Robert Rodriguez, also features a who's who of actors including Danny Trejo in the title role, as well as Carlos Estevez (better known as Charlie Sheen) playing the president of the United States. The film boasts appearances from Vanessa Hudgens, Sofia Vergara, Mel Gibson, Antonio Banderas, Jessica Alba, Amber Heard and Michelle Rodriguez.

"Machete Kills," the sequel to 2010's "Machete," opens in theaters on September 13, and marks Gaga's big debut on the big screen. And three weeks before she premieres the flick, she'll take the stage at the 2013 MTV Video Music Awards, where she will perform her new single, "Applause," off her November 11 album release, ARTPOP.

Gaga will release the song and video for [article id="1711560"]"Applause" on August 19[/article], giving her Little Monsters just enough time to learn all the words before she performs it live at the Barclays Center in Brooklyn at the VMAs on August 25.