Madonna is back in action, but this time she's mooning the audience instead of flashing her breasts.

Late last week, while performing "Human Nature" during her sold-out concert at Istanbul, Turkey's Türk Telekom Arena, the Queen of Pop stripped down to some lacy lingerie before pulling down her bra to expose her right breast.

The flash had everyone talking. Was it appropriate? Didn't she just say that nipple-flashing was kind of passé? Why is everyone surprised? This is Madonna after all.

Never one to bow down to her critics, and perhaps keen to keep the all the attention she's received in the wake of her own miniature nipple-gate, the 53-year-old pop star decided to bare even more (insanely toned) flesh at her concert Tuesday night in Rome, pulling down her pants to show off her bare(ish) bottom.

It happened, once again, during "Human Nature," as she stripped off her microphone pack, unbuttoned her striped black trousers, turned her back to the crowd, and exposed her thong-and-fishnet clad backside to the audience at the city's Stadio Olimpico.

If video from the show is any indication, the audience enjoyed this flash of flesh as much as it loved her first-round of MDNA Tour overexposure.

Madonna is certainly lining up the headlines on her latest road show. Her decision to mash up her classic "Express Yourself" with Lady Gaga's "Born this Way," presumably so we all continue to marvel at how similar the two songs sound, has inspired much conversation — including a comment from Gaga herself, who told the crowd during a recent concert in New Zealand, "It sometimes makes people feel better about themselves to put other people down or make fun of them or maybe make mockery of their work."

She has also angered French National Front leader Marine Le Pen by including an image of the conservative politician with a swastika on her forehead in the "Nobody Knows Me" video interlude shown during her show. Le Pen has threatened legal action if Madonna includes the image in either of her MDNA Tour shows in France, which are set for the country's national stadium, the Stade de France, in Paris on July 14 and at Nice's Stade Charles-Ehrmann on August 21.

The August 21 Nice show is the last on the European leg of Madonna's tour. She returns Stateside on August 28 for a sold-out show at Philadelphia's Wells Fargo Center.

What do you think of Madonna's latest exposure? Let us know in the comments!