On Thursday at his tour opener in Las Vegas, Jay kicked an a cappella line where he mentioned some of his past foes, as well as former friends.
"I ain't talking about gossip/ I ain't talking about Game," Hov rapped. "I ain't talking about Jimmy/ I ain't talking about Dame ... Dame made millions/ Even Jaz made some scraps/ He could've made more, but he ain't sign his contract."
Jim Jones and Game responded on the Fourth of July via their Twitter accounts.
"Question: if someone says in the song 'I'm not talkin' bout Jimmy,' is that not talkin' bout Jimmy? LOL," Jones tweeted. "We are back in business, people."
"Aight so, after about 10,000 responses ... The results read that he ain't dissin me," Game tweeted. "And as bad as I want to respond to his old a--, he can slide."
However, apparently Game changed his mind. In video footage that has been posted online, Game is seen calling out Hov onstage at a concert in France.
"We ain't come to play no games today," Game rapped. "We in Bordeaux, France ... F--- Jay-Z, if you don't like that, you can find an exit somewhere."
Game then leads the crowd in an obscene chant against Hov.
Game has both given props to and dissed Jay publicly many times before. Hov has never officially responded to him in song, although many inferred that several lines in the original "Dear Summer" freestyle Jay spit several years ago on Funk Master Flex's radio show ("You saying my name to entertain your crew/ I ain't playing no games, sh--, the game's through ... You playing a game you know you can't win/ Quit playing them childish games with grown men") were directed at the Compton MC.