James Stewart (Tom Destry)
Marlene Dietrich (Frenchy)
Charles Winninger (Wash Dimsdale)
Mischa Auer (Boris Callahan)
Brian Donlevy (Kent)
Harry Tenbrook (Stage Rider)
William Steele
Bob McKenzie (Doctor)
Philo McCullough (Bartender)
Ann E. Todd (Claggett Girl)
Minerva Urecal (Mrs. DeWitt)
Lillian Yarbo (Clara)
Lloyd Ingraham (Turner, the Express Agent)
Dick Jones (Eli Whitney Claggett)
Loren B. Brown (Juggler)
Harold DeGarro (Juggler)
Edmund MacDonald (Rockwell)
Bud McClure (Stage Driver)
Billy Bletcher (Pianist)
Virginia Brissac (Sophie Claggett)
Chief John Big Tree (Indian in Saloon)
Dora Clement (Woman)
Bill Cody, Jr. (Small Boy)
Harry Cording (Rowdy)
Carmen D'Antonio (Dancer)
Richard Alexander (Cowboy)
Joseph King (Sheriff Keogh)
Tom Fadden (Lem Claggett)
Samuel S. Hinds (Judge Hiram Slade)
Billy Gilbert (Loupqerou, the Bartender)
Jack Carson (Jack Tyndall)
Irene Hervey (Janice Tyndall)
Allen Jenkins (Gyp Watson)
Una Merkel (Lily Belle Callahan)
Warren Hymer (Bugs Watson)