Bette Davis (Elizabeth I)
Errol Flynn (Robert Devereaux, Earl of Essex)
Olivia de Havilland (Penelope Gray)
Vincent Price (Sir Walter Raleigh)
Donald Crisp (Francis Bacon)
Alan Hale (Earl of Tyrone)
Maris Wrixon
Rosella Towne
John Sutton
Judith Anderson
Doris Lloyd
I. Stanford Jolley
Forrester Harvey
Guy Bellis (Lord Charles Howard)
Nanette Fabray (Mistress Margaret Radcliffe)
Ralph Forbes (Lord Knollys)
Robert Warwick (Lord Mountjoy)
Leo G. Carroll (Sir Edward Coke)
Henry Stephenson (Lord Burghley)
Henry Daniell (Sir Robert Cecil)
James Stephenson (Sir Thomas Egerton)